Work

Optimized design and data analysis of tag-based cytosine methylation assays

Year: 2010

Type: article

Abstract: Using the type III restriction-modification enzyme EcoP15I, we isolated sequences flanking sites digested by the methylation-sensitive HpaII enzyme or its methylation-insensitive MspI isoschizomer for massively parallel sequencing. A novel data transformation allows us to normalise HpaII by MspI counts, resulting in more accurate quantification of methylation at >1.8 million loci in the human genome. This HELP-tagging assay is not sensitive to sequence polymorphism or base composition and allows exploration of both CG-rich and depleted genomic contexts.

Source: Genome biology

DOI: 10.1186/gb-2010-11-4-r36

Authors: Masako Suzuki, Qiang Jing, Daniel Lia, Marién Pascual, Andrew McLellan, John M Greally

Institution: Albert Einstein College of Medicine

Language: English

Topic: Epigenetics and DNA Methylation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $5,490

Funders: National Institutes of Health, National Human Genome Research Institute