Work

Preparative Isolation of Isorhamnetin from Stigma Maydis using High Speed Countercurrent Chromatography

Year: 2008

Type: article

Abstract: Preparative high-speed countercurrent chromatography (HSCCC) has been successfully used for the isolation and purification of isorhamnetin from Stigma maydis. This was achieved in two stages: the first separation was performed with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (HEMW) at a volume ratio of 5:5:5:5, yielding isorhamnetinat 65.6 %, which is followed by the second run using a two-phase solvent system composed of HEMW 5:5:6:4, v/v. From 700 mg of the crude extract 11.8 mg of isorhamnetin was obtained at a high purity of 98%. The final identification was performed by MS, (1)H-NMR and (13)C-NMR spectra.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826070802603369

Authors: Xiaoying Cao, Yun Wei, Yoichiro Ito

Institutions: Beijing University of Chemical Technology, National Institutes of Health, National Heart, Lung, and Blood Institute

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009