Year: 2010
Type: article
Abstract: AMP-activated protein kinase (AMPK) is an energy sensor essential for maintaining cellular energy homeostasis. Here, we report that AMPKalpha1 is the predominant isoform of AMPK in murine erythrocytes and mice globally deficient in AMPKalpha1 (AMPKalpha1(-/-)), but not in those lacking AMPKalpha2, and the mice had markedly enlarged spleens with dramatically increased proportions of Ter119-positive erythroid cells. Blood tests revealed significantly decreased erythrocyte and hemoglobin levels with increased reticulocyte counts and elevated plasma erythropoietin concentrations in AMPKalpha1(-/-) mice. The life span of erythrocytes from AMPKalpha1(-/-) mice was less than that in wild-type littermates, and the levels of reactive oxygen species and oxidized proteins were significantly increased in AMPKalpha1(-/-) erythrocytes. In keeping with the elevated oxidative stress, treatment of AMPKalpha1(-/-) mice with the antioxidant, tempol, resulted in decreased reticulocyte counts and improved erythrocyte survival. Furthermore, the expression of Foxo3 and reactive oxygen species scavenging enzymes was significantly decreased in erythroblasts from AMPKalpha1(-/-) mice. Collectively, these results establish an essential role for AMPKalpha1 in regulating oxidative stress and life span in erythrocytes.
Source: Journal of Biological Chemistry
Authors: Shaobin Wang, George L. Dale, Ping Song, Benoit Viollet, Ming-hui Zou
Language: English
Subfield: Physiology
Field: Medicine
Domain: Health Sciences
Open Access status: hybrid
Estimated APC paid: $3,430
Awards: G1148458614, Prevention of High Fat Diet-Induced Vascular Injury, G2512249395, G2558624076, Autophagy and diabetic vascular diseases, G3661356095, G3955034873, G408709736, G5186065327, G5369843531, G538943195, G5607009061, G5653212368, G6532516498, G665508143, G6884235968, G788250836, Inflammasome and unstable atherosclerotic plaques, G867234492, Angiotensin-II, GTPCH1 and 26S Protesomes, G8984010079, G9000212558, G9000284892
OpenAlex ID: W2033898418