Work

A highly sensitive chemiluminescence assay for superoxide detection and chronic granulomatous disease diagnosis

Year: 2011

Type: article

Abstract: Reactive oxygen species (ROS) produced by neutrophils are crucial for defense against infectious diseases, and the adequate measurement of ROS levels is an important way to evaluate the possibility of infections. The fluorescent probe dihydrorhodamine 123 has been applied exclusively to the measurement of ROS thus far. We developed a novel method for detecting ROS, which utilizes the chemiluminescent probes Luminol and Diogenes. The new method quantitatively detects ROS produced by as few as 10 to 10(4) neutrophils. Furthermore, this method can detect ROS levels in one microliter of whole blood or ROS produced by Epstein-Barr immortalized B lymphocytes. This method will be valuable for prompt diagnosis of neonatal chronic granulomatous diseases in which neutrophils aberrantly produce superoxide.

Source: Tropical Medicine and Health

DOI: 10.2149/tmh.2011-08

Authors: Tsuyoshi Yamazaki, Chikage Kawai, Akira Yamauchi, Futoshi Kuribayashi

Institutions: Tokai University, Kawasaki Medical School

Language: English

Topic: Neutrophil, Myeloperoxidase and Oxidative Mechanisms

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Ministry of Education, Culture, Sports, Science and Technology, Kawasaki Medical School, Japan Society for the Promotion of Science