Work

Association of 2' - 5' oligoribonucleotides

Year: 1992

Type: article

Abstract: Oligoribonucleotides with 2'-5' linkages have been synthesized on solid support. UV melting and CD experiments indicate complementary strands associate to give complexes with melting temperatures 30 to 40 degrees C lower than for duplexes formed by 3'-5' oligoribonucleotides with the same sequence. UV melting and imino proton NMR spectra and NOEs for (2'-5') CGGCGCCG are consistent with formation of an antiparallel duplex. The results suggest greater duplex stability was one factor favoring 3'-5' over 2'-5' linkages in evolution.

Source: Nucleic Acids Research

DOI: 10.1093/nar/20.7.1685

Authors: Ryszard Kierzek, Liyan He, Douglas H. Turner

Institutions: Institute of Bioorganic Chemistry, Polish Academy of Sciences, University of Rochester

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Folding RNA: from Aids to the Human Genome