Work

CAT2-mediated L-arginine transport and nitric oxide production in activated macrophages

Year: 1999

Type: article

Abstract: Activated macrophages require l-arginine uptake to sustain NO synthesis. Several transport systems could mediate this l-arginine influx. Using competition analysis and gene-expression studies, amino acid transport system y+ was identified as the major carrier responsible for this activity. To identify which of the four known y+ transport-system genes is involved in macrophage-induced l-arginine uptake, we used a hybrid-depletion study in Xenopus oocytes. Cationic amino acid transporter (CAT) 2 antisense oligodeoxyribonucleotides abolished the activated-macrophage-mRNA-induced l-arginine transport. Together with expression studies documenting that CAT2 mRNA and protein levels are elevated with increased l-arginine uptake, our data demonstrate that CAT2 mediates the l-arginine transport that is required for the raised NO production in activated J774 macrophages.

Source: Biochemical Journal

DOI: 10.1042/0264-6021:3400549

Authors: Donald K. KAKUDA, Matthew J. SWEET, Carol L. MACLEOD, David A. HUME, Daniel MARKOVICH

Institutions: The University of Queensland, University of California San Diego

Language: English

Topic: Amino Acid Enzymes and Metabolism

Subfield: Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Susan G. Komen for the Cure, Medical Research Council, Australian Research Council, National Health and Medical Research Council

OpenAlex ID: W4230793414