Work

Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators

Year: 2010

Type: article

Abstract: High affinity capture agents recognizing biomolecular targets are essential in the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously determining kinetic association and dissociation constants for two representative protein capture agents: a thrombin-binding DNA aptamer and an anti-thrombin monoclonal antibody. The scalability and inherent multiplexing capability of the technology make it an attractive platform for simultaneously evaluating the binding characteristics of multiple capture agents recognizing the same target antigen, and thus a tool complementary to emerging high-throughput capture agent generation strategies.

Source: The Analyst

DOI: 10.1039/c0an00853b

Authors: Ji-Yeon Byeon, Ryan C. Bailey

Institution: University of Illinois Urbana-Champaign

Language: English

Topic: Photonic and Optical Devices

Subfield: Electrical and Electronic Engineering

Field: Engineering

Domain: Physical Sciences

Open Access status: green

Funders: National Science Foundation, National Institutes of Health

Award: Personalized Clinical Diagnostics and Beyond: Integrated Ring Resonator Arrays