Work

Isolation of monoclonal antibodies that react preferentially with Listeria monocytogenes

Year: 1991

Type: article

Abstract: We have isolated and characterized three monoclonal antibodies that preferentially recognize the pathogen Listeria monocytogenes. Mouse monoclonal antibodies were produced by fusion of spleen cells from a Balb/c mouse, which was immunized with live L. monocytogenes, to an NS-1 myeloma partner. Hybridomas that produced antibodies which preferentially reacted with L. monocytogenes were identified by a direct binding enzyme-linked immunoabsorbent assay (ELISA) using a panel of bacteria which included L. ivanovii, Streptococcus faecalis, L. seeligeri, L. innocua and L. monocytogenes Scott A. Three hybridomas, Mab 20-10-2, Mab 36-6-12 and Mab 56-9-16 which preferentially reacted with L. monocytogenes were selected for further analysis. An indirect ‘sandwich’ ELISA was developed and used to further confirm that these hybridomas reacted with a number of L. monocytogenes isolates and not with other common cross-reacting bacteria including Kurthia sp.

Source: Food Microbiology

DOI: 10.1016/s0740-0020(05)80006-1

Authors: Jerrie Gavalchin, Mary L. Tortorello, Renae Malek, Margaret Landers, Carl A. Batt

Institutions: SUNY Upstate Medical University, Cornell University, New York State College of Agriculture & Life Sciences

Language: English

Topic: Listeria monocytogenes in Food Safety

Subfield: Biotechnology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,210