Work

Bafilomycins: a class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells.

Year: 1988

Type: article

Abstract: Various membrane ATPases have been tested for their sensitivity to bafilomycin A1, a macrolide antibiotic. F1F0 ATPases from bacteria and mitochondria are not affected by this antibiotic. In contrast, E1E2 ATPases--e.g., the K+-dependent (Kdp) ATPase from Escherichia coli, the Na+,K+-ATPase from ox brain, and the Ca2+-ATPase from sarcoplasmic reticulum--are moderately sensitive to this inhibitor. Finally, membrane ATPases from Neurospora vacuoles, chromaffin granules, and plant vacuoles are extremely sensitive. From this we conclude that bafilomycin A1 is a valuable tool for distinguishing among the three different types of ATPases and represents the first relatively specific potent inhibitor of vacuolar ATPases.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.85.21.7972

Authors: E J Bowman, A Siebers, K Altendorf

Institutions: University of California, Santa Cruz, Osnabrück University

Language: English

Topic: ATP Synthase and ATPases Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: Deutsche Forschungsgemeinschaft, National Institutes of Health, National Institute of General Medical Sciences

Awards: G1319549636, EUKARYOTIC VACUOLAR ATPASE