Work

Synthetic Enantiopure Carbohydrate Polymers That Are Highly Soluble in Water and Noncytotoxic

Year: 2013

Type: article

Abstract: The first synthesis of poly-amido-saccharides (PASs) from a galactose(gal)-derived β-lactam sugar monomer is reported. The polymers are prepared using a controlled anionic ring-opening polymerization and characterized by NMR, optical rotation, IR, and GPC. Galactose-derived PASs display high solubility in aqueous solutions and are noncytotoxic to HepG2, CHO, and HeLa cell lines. To evaluate whether gal-derived PASs are recognized by the gal-specific lectin present on human hepatocytes, cellular uptake of rhodamine-labeled polymers is assessed using flow cytometry and fluorescence microscopy. Based on these results, the polymers are taken into cells via endocytosis that is not dependent on the gal-specific receptor on hepatocytes. Neutral, hydrophilic polymers, such as gal-derived PASs, are desirable materials for a range of biomedical applications, such as drug delivery, surface passivation, and hydrogel formation.

Source: ACS Macro Letters

DOI: 10.1021/mz400394r

Authors: Eric L. Dane, Stacy L. Chin, Mark W. Grinstaff

Institution: Boston University

Language: English

Topic: biodegradable polymer synthesis and properties

Subfield: Biomaterials

Field: Materials Science

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: Synthesis and Evaluation of Antibacterial Anionic Dendritic Amphiphiles