Work

Complement (C5-a)-induced granulocyte aggregation in vitro. A possible mechanism of complement-mediated leukostasis and leukopenia.

Year: 1977

Type: article

Abstract: Activated plasma complement will induce biphasic aggregation of human granulocytes dectable by standard nephelometric techniques. The responsible active component was suggested to be C5a by molecular weight and heat-stability assays; moreover, aggragating activity was ablated by anti-C5 but not anti-C3 antibodies. C5a prepared by trypsinization of purified C5 reproduced the aggregating activity of whole activated plasma, whereas plasma from a C5-deficient donor did not support aggregation. Embolization of granulocyte aggregates might be a previously unsuspected cause of leukostasis and pulmonary damage in various clinical situations where intravascular complement activation occurs.

Source: Journal of Clinical Investigation

DOI: 10.1172/jci108763

Authors: P R Craddock, D Hammerschmidt, J G White, A P Dalmosso, H S Jacob

Institutions: University of Minnesota, United States Department of Veterans Affairs, Minneapolis VA Health Care System, Minneapolis VA Medical Center

Language: English

Topic: Complement system in diseases

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2036068566

FWCI: 10.47

Cites: 14

Cited by: 687

Related to: 10