Work

Two-photon excited cellular autofluorescence induced by cw and femtosecond NIR microradiation

Year: 1996

Type: conference-paper

Abstract: We report the first two-photon excited autofluorescence measurements in single cells induced by continuous wave NIR laser microbeams. In particular we demonstrate NIR-excited NAD(P)H fluorescence of motile spermatozoa in a single-beam gradient force optical trap ('optical tweezers'). In addition, two-photon NIR excited autofluorescence imaging and 'tau- mapping' of CHO cells was performed with laser scanning microradiation of a tunable femtosecond-Ti:sapphire laser. Cellular response on autofluorescence to two-photon absorption of NIR radiation was compared with exposure to UVA light (one-photon absorption).

Source: Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE

DOI: 10.1117/12.229988

Authors: Karsten Koenig, Peter T. C. So, William W. Mantulin, Enrico Gratton, Tatiana B. Krasieva, Michael W. Berns, Bruce J. Tromberg

Institutions: Beckman Laser Institute and Medical Clinic, University of Illinois Urbana-Champaign, University of California, Irvine

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2036669901

FWCI: 2.167

Cites: 4

Cited by: 1

Related to: 10