Work

Selective labeling of polypeptides using protein farnesyltransferase via rapid oxime ligation

Year: 2010

Type: article

Abstract: An aldehyde-containing alternative substrate for protein farnesyltransferase was prepared and shown to be enzymatically incorporated into a peptide and a protein. The protein was subsequently immobilized onto aminooxy-functionalized agarose beads or labeled with a fluorophore. This method for protein modification provides an alternative to the commonly employed Cu(I)-catalyzed click reaction.

Source: Chemical Communications

DOI: 10.1039/c0cc03305g

Authors: Mohammad Rashidian, Jonathan K. Dozier, Stepan Lenevich, Mark D. Distefano

Institution: University of Minnesota

Language: English

Topic: Click Chemistry and Applications

Subfield: Organic Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Awards: Enzymatic Protein labeling, Mechanism and function of protein prenylation, Chemistry-Biology Interface Training Grant