Work

In situ activation of bis-dialkylaminophosphines—a new method for synthesizing deoxyoligonucleotides on polymer supports

Year: 1984

Type: article

Abstract: Deoxynucleoside phosphoramidites can be prepared in good yield from deoxynucleosides, bis- dialkylaminophosphines , and the corresponding dialkylamine hydrotetrazolide or tetrazole as catalysts. These phosphoramidites generated in situ lead to the direct synthesis of deoxyoligonucleotides on polymer supports.

Source: Nucleic Acids Research

DOI: 10.1093/nar/12.10.4051

Authors: A.D. Barone, J.-Y. Tang, M.H. Caruthers

Institution: University of Colorado Boulder

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Institutes of Health, National Institute of General Medical Sciences

Award: CHEMICAL AND ENZYMATIC STUDIES WITH SYNTHETIC DNA/RNA