Work

Sequence and structure correlation of human ribosomal transcribed spacers

Year: 1990

Type: article

Abstract: We report the sequences of the transcribed spacers of human rRNA that now allow us to piece together the entire primary transcript sequence of approximately 13.3 x 10(3) base-pairs. Comparison of transcribed spacer sequences with those of variable regions of rRNA and with those of the non-transcribed spacers supports the hypothesis that the variable regions are descended from transcribed spacers. Nucleotide sequence-derived secondary structures for the 5' external transcribed spacer and for internal transcribed spacers 1 and 2 match both the sizes and shapes of the structures that were visualized 15 years ago on electron micrographs. Parts of these structures are conserved in mammals and may be related to transcript processing.

Source: Journal of Molecular Biology

DOI: 10.1016/0022-2836(90)90302-3

Authors: Iris Laudien Gonzalez, Carolyn Chambers, Jerome L. Gorski, Dwight Stambolian, Roy D. Schmickel, James E. Sylvester

Institutions: Hahnemann University Hospital, University of Pennsylvania

Language: English

Topic: RNA and protein synthesis mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,730

Funders: University of Pennsylvania, National Institutes of Health, National Human Genome Research Institute