Work

Biosynthesis and turnover of anandamide and other N‐acylethanolamines in peritoneal macrophages

Year: 1999

Type: article

Abstract: Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE). Addition of exogenous ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid. Incorporation of (18)O from H2 (18)O-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids.

Source: FEBS Letters

DOI: 10.1016/s0014-5793(99)01226-0

Authors: Toyoyasu Kuwae, Yasuo Shiota, Patricia C. Schmid, Randy Krebsbach, Harald H.O. Schmid

Institutions: Josai University, University of Minnesota

Language: English

Topic: Cannabis and Cannabinoid Research

Subfield: Pharmacology

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: Hormel Foundation, National Institutes of Health, National Institute of General Medical Sciences