Work

Comparative NMR analysis of the decadeoxynucleotide d-(GCATTAATGC)2and an anlogue containing 2-aminoadenine

Year: 1991

Type: article

Abstract: The dodecadeoxynucleotide duplex d-(GCATTAATGC)2 has been prepared with all adenine bases replaced by 2-NH2-adenine. This modified duplex has been characterized by nuclear magnetic resonance (NMR) spectroscopy. Complete sequence-specific 1H resonance assignments have been obtained by using a variety of 2D NMR methods. Multiple quantum-filtered and multiple quantum experiments have been used to completely assign all sugar ring protons, including 5'H and 5'H resonances. The assignments form the basis for a detailed comparative analysis of the 1H NMR parameters of the modified and parent duplex. The structural features of both decamer duplexes in solution are characteristic of the B-DNA family. The spin-spin coupling constants in the sugar rings and the relative spatial proximities of protons in the bases and sugars (as determined from the comparison of corresponding nuclear Overhauser effects) are virtually identical in the parent and modified duplexes. Thus, substitution by this adenine analogue in oligonucleotides appears not to disturb the global or local conformation of the DNA duplex.

Source: Nucleic Acids Research

DOI: 10.1093/nar/19.20.5507

Authors: Walter J. Chazin, Mark Rance, Andrè Chollet, Werner Luepin

Institutions: Scripps Research Institute, Roche (Switzerland)

Language: English

Topic: DNA and Nucleic Acid Chemistry

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: National Science Foundation, National Institutes of Health, National Institute of General Medical Sciences

Awards: Development of NMR Methods for Studies of Biomolecules, G4426997795, METHODOLOGY DEVELOPMENT FOR NMR STUDIES OF BIOMOLECULES