Year: 2014
Type: article
Abstract: In the adult subventricular zone (neurogenic niche), neural stem cells double-positive for two markers of subsets of neural stem cells in the adult central nervous system, glial fibrillary acidic protein and CD133, lie in proximity to fractones and to blood vessel basement membranes, which contain the heparan sulfate proteoglycan perlecan. Here, we demonstrate that perlecan deficiency reduces the number of both GFAP/CD133-positive neural stem cells in the subventricular zone and new neurons integrating into the olfactory bulb. We also show that FGF-2 treatment induces the expression of cyclin D2 through the activation of the Akt and Erk1/2 pathways and promotes neurosphere formation in vitro. However, in the absence of perlecan, FGF-2 fails to promote neurosphere formation. These results suggest that perlecan is a component of the neurogenic niche that regulates FGF-2 signaling and acts by promoting neural stem cell self-renewal and neurogenesis.
Source: Stem Cell Research
Authors: Aurelien Kerever, Frederic Mercier, Risa Nonaka, Susana de Vega, Yuka Oda, Bernard Zalc, Yohei Okada, Nobutaka Hattori, Yoshihiko Yamada, Eri Arikawa-Hirasawa
Institutions: Juntendo University, University of Hawaiʻi at Mānoa, University of Hawaii System, Centre National de la Recherche Scientifique, Inserm, Institut du Cerveau, Université Pierre-et-Marie-Curie, Keio University, National Institutes of Health, National Institute of Dental and Craniofacial Research
Language: English
Subfield: Developmental Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,360
OpenAlex ID: W2037940280