Work

Use of bacteria for rapid, pH-neutral, hydrolysis of the model hydrophobic carboxylic acid esterp-nitrophenyl picolinate

Year: 2012

Type: article

Abstract: Caulobacter crescentus, Escherichia coli and Bacillus subtilis cultures promote the hydrolysis of the model ester p-nitrophenyl picolinate (PNPP) at neutral pH with high efficiency. Hydrolysis is related to cell concentration, while the interaction of PNPP with both bacterial cells and their extracellular molecules is required for a maximum rate of PNPP hydrolysis in C. crescentus cultures. Furthermore, C. crescentus cultures hydrolyze PNPP at concentrations useful in synthetic chemistry.

Source: Biocatalysis and Biotransformation

DOI: 10.3109/10242422.2012.702269

Authors: Alexandra E. Forest, Gordon G. Goldstine, Yann Schrodi, Sean R. Murray

Institution: California State University, Northridge

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, California State University, Northridge, National Institute of General Medical Sciences

Award: Integrating lipid biosynthesis with bacterial cell cycle progression