Work

Full Developmental Potential of Mammalian Preimplantation Embryos is Maintained After Imaging Using a Spinning-Disk Confocal Microscope

Year: 2006

Type: article

Abstract: Fluorescent live imaging of cells and embryos at subcellular resolution poses significant challenges for biologists due to morbidity and mortality ensuing from phototoxicity. Here we report the use of a spinning-disk confocal microscope to image mouse and bovine preimplantation embryos without impairing their developmental potential. We also present data indicating that this imaging technique does not affect the functionality of subcellular components as assessed by reactive oxygen species (ROS) production, caspase activity, and DNA integrity. Spinning-disk confocal microscopy was also useful in determining cell number and allocation in transgenic bovine blastocysts. We conclude that this imaging method is suitable for monitoring preimplantation embryos.

Source: BioTechniques

DOI: 10.2144/000112310

Authors: Pablo Juan Ross, Gloria Ines Perez, Tak Ko, Myung Sik Yoo, Jose Bernardo Cibelli

Institution: Michigan State University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,415

Funder: Michigan State University