Alterations of T‐cell surface markers in older women with persistent human papillomavirus infection
Year: 2010
Type: article
Study design: Observational Study
Abstract: We previously reported decreased lymphocyte proliferative responses among older women with persistent human papillomavirus (HPV) infection. To characterize the phenotype of peripheral lymphocytes associated with persistent HPV infection, we evaluated the expression of different cell surface markers in peripheral blood mononuclear cells (PBMCs) from a case-control study within a 10,049 woman population-based cohort study in Guanacaste, Costa Rica. Women in the cohort aged 46-74 and with HPV results at their 5th year anniversary visit were considered, and all women (n = 87) with persistent HPV infections, all women (n = 196) with transient HPV infections and a random sample of HPV DNA-negative women (n = 261) frequency-matched to cases on age were selected for this study. A median of 3 years after the case-control matching visit, cervical cells were collected for liquid-based cytology and repeat HPV DNA genotyping. Blood was obtained from which PBMCs were extracted and cryopreserved for immunological phenotyping via flow cytometry. Significant increases in risk of HPV persistence were observed for 3 marker subsets indicative of immune cell activation/differentiation. Relative risk estimates were 5.4 (95% CI = 2.2-13.3) for CD69(+)CD4(+), 2.6 (95% CI = 1.2-5.9) for HLADR(+)CD3(+)CD4(+) and 2.3 (95% CI = 1.1-4.7) for CD45RO(+)CD27(-)CD8(+). A significant decrease in HPV persistence was observed for a subset marker indicative of an immature, undifferentiated memory state CD45RO(+)CD27(+)CD4(+) (OR = 0.36; 95% CI = 0.17-0.76). Adjustment for these markers only partially explained the previously reported association between decreased lymphoproliferative responses and persistent HPV infection. Whether phenotypic alterations observed predispose to HPV persistence or result from it should be the focus of future studies.
Source: International Journal of Cancer
DOI: 10.1002/ijc.25371
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: green
Awards: G113982338, G1356092923, G1519249481, DCTD Computer Support, G1799052500, POPULATION-BASED NATURAL HISTORY OF CERVICAL NEOPLASIA, G2012962634, G2116629794, G2301432094, POPULATION BASED NATURAL HISTORY OF CERVICAL NEOPLASIA, PERSISTENT HPV IN WOMEN AT RISK FOR CERVIX CANCER, G354811447, Persistent HPV Infection in Women at Risk for Cervix Cancer, G420340792, POPULATION-BASED NATURAL HISTORY OF CERVICAL NEOPLASIA, POPULATION-BASED NATURAL HISTORY OF CERVICAL NEOPLASIA, BIOMEDICAL COMPUTING SUPPORT FOR THE DIVISION OF CANCER EPIDEMIOLOGY AND GENETIC, GUANACASTE PROJECT--A POPULATION BASED NATURAL HIST, G816316942, HPV, cervical neoplasia, and vaccination
OpenAlex ID: W2038386404