Work

Single-strand DNA intermediates in phage λ’s Red recombination pathway

Year: 1997

Type: article

Abstract: An assay was developed to assess early intermediates arising in lambda's Red recombination pathway. Double-strand breaks were delivered in vivo to nonreplicating lambda chromosomes. Analysis by blot hybridization of total DNA extracts revealed the following: (i) long (>1.4 kilobases) single-strand DNA (ssDNA) intermediates; (ii) resection proceeding bidirectionally from the break site; (iii) single-strand overhangs of 3' polarity; and (iv) in the absence of lambda's ninR functions, a requirement of the red alpha gene product for the production of ssDNA. Therefore, the physical characteristics exhibited by these ssDNA molecules are consistent with their being an early recombination intermediate in the Red recombination pathway as proposed previously from genetic and in vitro biochemical analyses.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.94.7.2951

Authors: Stuart A. Hill, Mary M. Stahl, Franklin W. Stahl

Institutions: National Institutes of Health, University of Oregon, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories

Language: English

Topic: DNA Repair Mechanisms

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of General Medical Sciences

Award: G2004666982