Work

In vivo neurochemical profiling of rat brain by 1H‐[13C] NMR spectroscopy: cerebral energetics and glutamatergic/GABAergic neurotransmission

Year: 2009

Type: article

Abstract: The quantification of excitatory and inhibitory neurotransmission and the associated energy metabolism is crucial for a proper understanding of brain function. Although the detection of glutamatergic neurotransmission in vivo by (13)C NMR spectroscopy is now relatively routine, the detection of GABAergic neurotransmission in vivo has remained elusive because of the low GABA concentration and spectral overlap. Using (1)H-[(13)C] NMR spectroscopy at high magnetic field in combination with robust spectral modeling and the use of different substrates, [U-(13)C(6)]-glucose and [2-(13)C]-acetate, it is shown that GABAergic, as well as glutamatergic neurotransmitter fluxes can be detected non-invasively in rat brain in vivo.

Source: Journal of Neurochemistry

DOI: 10.1111/j.1471-4159.2009.06428.x

Authors: Pieter Van Eijsden, Kevin L. Behar, Graeme F. Mason, Kees P. J. Braun, Robin A. De Graaf

Institutions: Utrecht University, University Medical Center Utrecht, Yale University

Language: English

Topic: Advanced MRI Techniques and Applications

Subfield: Radiology, Nuclear Medicine and Imaging

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: bronze

Funders: National Institutes of Health, National Institute on Alcohol Abuse and Alcoholism, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Neurological Disorders and Stroke