Work

An immortalized cell line expresses properties of activated microglial cells

Year: 1992

Type: article

Abstract: Murine cultured microglial cells were immortalized after infection with a v-raf/v-myc recombinant retrovirus. This immortalized cell line (BV-2) shares properties with body macrophages with respect to the antigen profile, their phagocytic capacity and antimicrobial activity. BV-2 cells are not constitutively able to kill tumor cells in vitro, but acquire antitumor activity following an increase in [Ca++]i. BV-2 cells, like microglial cells, are however, distinct from peripheral macrophages by their expression of inwardly rectifying K+ channels in concert with a lack in outwardly rectifying K+ channels and the formation of spineous processes. The BV-2 cell line thus represents a suitable model for in vitro studies of activated microglial cells.

Source: Journal of Neuroscience Research

DOI: 10.1002/jnr.490310405

Authors: V. Bocchini, R. Mazzolla, R. Barluzzi, E. Blasi, P. Sick, H. Kettenmann

Institutions: University of Perugia, Heidelberg University

Language: English

Topic: Neuroinflammation and Neurodegeneration Mechanisms

Subfield: Neurology

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: Eli Lilly and Company