Work

Demonstration of REBASE‐assisted restriction mapping to determine the recognition site of unknown restriction endonucleases

Year: 2007

Type: article

Abstract: An important step in the characterization of a new restriction enzyme involves determination of its recognition site. Comparison of its DNA substrate digestion fragment patterns with those obtained using enzymes of known specificity indicates whether the enzyme recognizes a novel sequence or is an isoschizomer of already existing prototype. REBASE (Restriction Enzyme dataBASE: http://www.neb.com/rebase)-assisted restriction mapping is described in this paper for a rare cutter [TspMI (REBASE No. 7191)] and a frequent cutter [BflI (REBASE No. 4910)] as a practical exercise for undergraduate students to understand how to determine recognition sequence of a REase.

Source: Biochemistry and Molecular Biology Education

DOI: 10.1002/bmb.82

Authors: Vijay Parashar, Neena Capalash, Prince Sharma

Institution: Panjab University

Language: English

Topic: Genetics, Bioinformatics, and Biomedical Research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2039399143

FWCI:

Cites: 8

Cited by: 1

Related to: 10