Work

Miniaturized side-viewing imaging probe for fluorescence lifetime imaging (FLIM): validation with fluorescence dyes, tissue structural proteins and tissue specimens

Year: 2007

Type: article

Abstract: We report a side viewing fibre-based endoscope that is compatible with intravascular imaging and fluorescence lifetime imaging microscopy (FLIM). The instrument has been validated through testing with fluorescent dyes and collagen and elastin powders using the Laguerre expansion deconvolution technique to calculate the fluorescence lifetimes. The instrument has also been tested on freshly excised unstained animal vascular tissues.

Source: New Journal of Physics

DOI: 10.1088/1367-2630/9/5/127

Authors: D S Elson, J A Jo, L Marcu

Institutions: Imperial College London, University of California, Davis, Texas A&M University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $2,610

Funders: National Science Foundation, National Heart, Lung, and Blood Institute