Work

Homo- and Hetero-Oligomerization in the Slc26a Protein Family

Year: 2011

Type: conference-paper

Abstract: The motor‐protein prestin is thought to be a homo‐oligomer, but the oligomerization motifs are unknown. We used the acceptor‐photobleach variant of fluorescence‐lifetime based FRET analysis to demonstrate that homo‐oligomerization is not only common to mammalian and non‐mammalian prestins, but occurs in widely divergent members of the Slc26a family proteins. We therefore tested the hypothesis that oligomerization is conserved across the Slc26a family by measuring FRET between different Slc26a family molecules. Our results show that hetero‐oligomerization is common between family members, which suggest that common oligomerization motifs exist.

Source: AIP conference proceedings

DOI: 10.1063/1.3658077

Authors: Benjamin Currall, Heather Jensen-Smith, Richard Hallworth, Christopher A. Shera, Elizabeth S. Olson

Institutions: Creighton University, Brigham and Women's Hospital, Harvard University

Language: English

Topic: Advanced Fluorescence Microscopy Techniques

Subfield: Biophysics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: Creighton University, St. Jude Children's Research Hospital, National Institutes of Health, National Center for Research Resources

Award: CONSTRUCTION OF RESEARCH FACILITY