Year: 1970
Type: article
Abstract: The steroid glucuronide, and mono- and disulphate fractions were obtained from human bile by chromatography on Sephadex LH-20. The conjugates in the glucuronide fraction were hyderolysed with β-glucuronidase and the mono- and disulphate fractions were solvolysed. The steroids liberated were fractionated on silicic acid and by thin layer chromatography and analysed by gas-liquid chromatography and gas chromatography-mass spectrometry. The following C21O3 and C21O4 steroids were identified: 5α-pregnane-3α,17α,20α-triol, 5β-pregnane-3α,17α,20α-triol, 5-pregnene-3α,17α,20α-triol, 5-pregnene-3β,17α,20α-triol, 5α-pregnane-3α,16α,20α-triol, 3α,21-dihydroxy-5β-pregnan-20-one, 3α,11β,21-trihydroxy-5α-pregnan-20-one, 3α,11β,21-trihydroxy-5β-pregnan-20-one and 3α,21-dihydroxy-5β-pregnane-11,20-dione. Concentrations of these steroids were measured in fistula or gall-bladder bile samples from five subjects.
Source: European Journal of Biochemistry
Author: Timo Laatikainen
Institution: University of Helsinki
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2039631419