Work

Estradiol induces rapid remodelling of plasma membranes in developing rat cerebrocortical neurons in culture

Year: 1989

Type: article

Abstract: Exo-endocytotic images and intramembrane particles were quantitatively assessed in freeze-fracture replicas from the plasma membrane of dispersed fetal rat cortical neurons (day 16 gestation) grown for 24 days in culture. The addition of 10(-10) M 17 beta-estradiol to the culture medium resulted in a significant increase in the numerical density of exo-endocytotic images within 1 min. A further increase of the number of exo-endocytotic images associated to a significant decrease in the number of intramembrane particles was observed in cells exposed for 10 min to 17 beta-estradiol. Similar results were observed when the cells were exposed to 17 beta-estradiol for 17 days. No effects on exo-endocytotic images and intramembrane particles were observed when 17 alpha-estradiol was added, instead of 17 beta-estradiol, to the cultures. These results indicate that physiological levels of 17 beta-estradiol can have rapid effects upon the ultrastructure of the neuronal membrane of developing cerebrocortical neurons.

Source: Brain Research

DOI: 10.1016/0006-8993(89)91113-x

Authors: L.M. Garcia-Segura, G. Olmos, R.J. Robbins, P. Hernandez, J.H. Meyer, F. Naftolin

Institutions: Instituto Cajal, Yale University

Language: English

Topic: Estrogen and related hormone effects

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,360