Work

Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5′ end of the chicken α-skeletal actin gene

Year: 1986

Type: article

Abstract: alpha-skeletal actin message levels have been shown to be tightly regulated in chicken primary myoblast cultures. To test for gene elements required for muscle cell specific expression, DNA sequences containing the 5'-flanking regions of the chicken alpha-skeletal actin, beta-cytoplasmic actin, and the histone H2b genes were linked to the coding sequences of the chloramphenicol acetyltransferase gene and transfected into myogenic and non-myogenic cells. In contrast to beta-actin CAT hybrids, the alpha-skeletal actin CAT constructions displayed restricted CAT expression in transfected non-myogenic cells. We showed that a 411 nucleotide fragment flanking the 5' end of of the alpha-skeletal actin gene was responsible for a 9-15 fold increase in CAT enzymatic activity during myoblast fusion, versus only a transient 2 fold rise for the beta-actin and histone flanking sequences. These results indicate that DNA sequences within 411 bp of the 5' terminus of the alpha-skeletal actin gene influenced its cell type and stage specific expression.

Source: Nucleic Acids Research

DOI: 10.1093/nar/14.4.1683

Authors: James M. Grichnik, Derk J. Bergsma, Robert J. Schwartz

Institution: Baylor College of Medicine

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: American Heart Association, Muscular Dystrophy Association, National Institute of Neurological Disorders and Stroke

Award: G612430876