Work

Isolation of the Xenopus nrp‐1 gene and analysis of its expression following the midblastula transition in injected Xenopus embryos

Year: 1996

Type: article

Abstract: During embryogenesis the differentiation of distinct tissues is marked by the expression of tissue-specific genes. In Xenopus, the neural-specific gene nrp-1 is activated following the midblastula transition (MBT), and is then expressed exclusively in developing neural tissue. To pursue an investigation of the transcriptional regulation of nrp-1, the genomic DNA encoding nrp-1 was isolated and its structure analyzed. Nrp-1 lacks a canonical TATA box proximal to the start site of transcription, but the 5' flanking sequence is highly GC-rich. Injection studies with nrp-1/chloramphenicol acetyltransferase and luciferase constructs demonstrate that in Xenopus embryos, 200 nt of the 5' flanking sequence of nrp-1 are sufficient to drive the expression of reporter genes soon after the MBT, albeit not in a tissue-specific manner. This expression is enhanced by the inclusion of larger regions of the nrp-1 gene.

Source: Development Growth & Differentiation

DOI: 10.1046/j.1440-169x.1996.t01-3-00012.x

Authors: Marcia L. O'Connell, Martha L. Rebbert, Igor B. Dawid

Institutions: National Institutes of Health, Eunice Kennedy Shriver National Institute of Child Health and Human Development, The College of New Jersey

Language: English

Topic: Developmental Biology and Gene Regulation

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

OpenAlex ID: W2039779129

FWCI: 0.127

Cites: 66

Cited by: 1

Related to: 10