Work

Cloned extrachromosomal circular DNA copies of the human transposable element THE-1 are related predominantly to a single type of family member

Year: 1987

Type: article

Abstract: The 2300 base-pair transposon-like human element, THE-1, has been identified in the extrachromosomal circular DNA of the established human cell line HeLa as a relatively homogeneous population of covalently closed 1900 base-pair molecules. THE-1, which has been classified tentatively as a retroviral-like transposable element (a retrotransposon), is present in the extrachromosomal circular DNA of African green monkey (BSC-1) and human lymphoblastoid (Jurkat) cell lines. The 1900 base-pair extrachromosomal elements isolated and cloned from HeLa cells (1) appear to contain only THE-1-specific nucleotide sequences, (2) are circularized versions of the linear chromosomal sequence, and (3) are related predominantly to a single, or single type of, family member.

Source: Journal of Molecular Biology

DOI: 10.1016/0022-2836(87)90687-5

Authors: Ravi Misra, Andy Shih, Mark Rush, Elsie Wong, Carl W. Schmid

Institutions: NYU Langone Health, New York University, University of California, Davis

Language: English

Topic: Chromosomal and Genetic Variations

Subfield: Plant Science

Field: Agricultural and Biological Sciences

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $4,730

Funder: National Institute of General Medical Sciences