Work

Analysis of an evolutionarily conserved antigenic site on mammalian cytochrome c using synthetic peptides.

Year: 1985

Type: article

Abstract: Two synthetic peptides inclusive of the NH2-terminal N-acetyl-Gly-Asp-Val-Glu tetrapeptide of mammalian cytochrome c (cyt c) were used in this study to address the question of whether mammals can respond immunologically to an evolutionarily conserved region of a protein. These peptides were assessed for their capacity (i) to act as immunogens for the production of anti-self cyt c antisera and (ii) to bind rabbit anti-rodent cyt c antibody. The findings from these studies indicate the existence of an immunogenic determinant in an evolutionarily conserved region of cyt c that contains residues 1-4. This determinant can induce anti-self cyt c antibodies whether presented as a peptide on a carrier protein or in the context of the intact molecule as polymerized mammalian cyt c.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.82.5.1508

Authors: R Jemmerson, P R Morrow, N R Klinman, Y Paterson

Institution: Scripps Clinic

Language: English

Topic: vaccines and immunoinformatics approaches

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: Arthritis Foundation, Damon Runyon Cancer Research Foundation, National Institutes of Health, National Institute of Allergy and Infectious Diseases