Effects of Caloric Restriction on the Expression of PGC-1 and PPARs mRNA in Liver of Otsuka Long-Evans Tokushima Fatty Rats
Year: 2006
Type: article
Abstract: Background: Gluconeogenesis is strongly stimulated during fasting and is aberrantly activated in diabetes mellitus.PPARγ-coactivator 1 (PGC-1) and Peroxisome proliferator -activated receptors (PPARs) costimulate the expression of key enzymes of gluconeogenetic pathway.This study was performed to evaluate the response to dietary caloric restriction (CR) on the PPARs and PGC-1 expression in liver of diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) rats.Methods: Diabetic OLETF rats (male, 24 weeks) and Long-Evans Tokushima Otsuka (LETO) rats (male, 24 weeks) were used in this study.Liver PPARs and PGC-1 mRNA, and blood glucose levels were investigated at 1, 2, and 3 weeks after the beginning of 30% CR.PPARs and PGC-1 mRNA were determined by RT-PCR and blood glucose levels were measured by spectrophotometric assay.Results: The liver PGC-1 mRNA expressions were increased to 19% in non-diabetic LETO rats but significant change was not observed in diabetic OLETF rats by 30% CR.The liver PPARγ mRNA expressions were not changed in non-diabetic LETO rats but increased to 23% in diabetic OLETF rats by 30% CR.The difference of PPARα and PPARβ mRNA expressions in liver of OLETF and LETO rats were not observed.Conclusion: The liver PPARγ and PGC-1 expression response to CR are altered in OLETF rats compared to in LETO rats.These findings suggested that PPARγ and PGC-1 expression control system altered in diabetic OLETF rat liver and altered PPARγ and PCG-1 expression may some roles on the aberrantly activated gluconeogenesis in diabetes mellitus.(J Kor Diabetes Assoc 30:161～169, 2006) ━━━━━━━━━━━━━━━━━━
Institutions: Chosun University, Chosun University Hospital
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W2040654334