Work

TGF-β1 treated murine dendritic cells are maturation resistant and down-regulate Toll-like receptor 4 expression

Year: 2004

Type: article

Abstract: OBJECTIVE: To explore the effects of transforming growth factor beta1 (TGF-beta1) on dendritic cells (DC). METHODS: Murine bone marrow cells were cultured with GM-CSF and TGF-beta1 to develop TGF-beta1-treated DC (TGFbeta-DC). Then they were stimulated by lipopolysaccharide (LPS). Their phenotypes were assessed by flow cytometry (FCM). The allogeneic stimulating capacity of DC was measured by mixed lymphocyte reaction (MLR) using BrdU ELISA method and IL-12p70 protein was detected by ELISA. The expression of Toll-like receptor 4 (TLR4) was analyzed by semi quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and FCM. RESULTS: Compared to immature DC (imDC) cultured by GM-CSF alone, the TGFbeta-DC express lower CD80, CD86, I-Ab and CD40. The TGFbeta-DC were resistant to maturation with LPS. Maturation resistance was evident from a failure to up-regulate co-stimulatory molecules (CMs), to stimulate larger T cells proliferation and to enhance secretion of IL-12p70. We also found that TGF-beta1 could down-regulate TLR4 expression on TGFbeta-DC. CONCLUSION: TGFbeta-DC are resistant to maturation stimulus (LPS) and might have some correlation with the down-modulation of TLR4 expression.

Source: Journal of Zhejiang University. Science A

DOI: 10.1631/jzus.2004.1239

Authors: Hai-bo Mou, Mao-fang Lin, Hong Cen, Jing Yu, Xiao-jian Meng

Institutions: First Affiliated Hospital Zhejiang University, Zhejiang University

Language: English

Topic: Immunotherapy and Immune Responses

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funder: National Natural Science Foundation of China