Work

ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell

Year: 2014

Type: article

Abstract: A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell.

Source: Journal of the American Society for Mass Spectrometry

DOI: 10.1007/s13361-014-0956-2

Authors: Valery G. Voinov, Samuel E. Bennett, Joseph S. Beckman, Douglas F. Barofsky

Institutions: Oregon State University, Pacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of Sciences

Language: English

Topic: Mass Spectrometry Techniques and Applications

Subfield: Spectroscopy

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funders: National Institutes of Health, National Institute of Environmental Health Sciences, National Center for Research Resources

Awards: G1497661912, A Universal Electromagnetostatic Tandem-Mass-Spectrometric Dissociation-Cell for, Reducing Susceptibility to Environmental Stress Throughout the Life Span