Work

Trapping and Characterization of Novel Retinoid Response Elements

Year: 2003

Type: article

Abstract: Retinoids, such as retinoic acid (RA), play a critical role in normal vertebrate development and physiology. However, embryonic exposure to excess retinoids also causes severe malformations. Retinoids bind RA receptors and retinoid X receptors, thus activating a plethora of genes. Separating the genes induced directly by retinoid-bound receptors from those induced subsequently by other transcription factors is difficult. The loose consensus defining known RA responsive elements (RAREs) further complicates this effort. We developed a yeast-based system to trap functional RAREs in the mouse genome. Several of the clones contain RAREs near RA-induced genes. Mammalian reporter gene analyses and EMSAs showed that these are bona fide RAREs. This functional genomics approach should identify RA-regulated genes that initiate critical signaling cascades in cells.

Source: Molecular Endocrinology

DOI: 10.1210/me.2002-0192

Authors: Michele A. Glozak, Yong Li, Rae Reuille, Kwan Hee Kim, My-Nuong Vo, Melissa B. Rogers

Institutions: University of South Florida, Washington State University, Rutgers New Jersey Medical School

Language: English

Topic: Retinoids in leukemia and cellular processes

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: bronze

Funders: March of Dimes Foundation, Moffitt Cancer Center, National Institute of Child Health and Human Development, Eunice Kennedy Shriver National Institute of Child Health and Human Development

Award: G2765735163