Year: 1999
Type: article
Abstract: A dichloromethane extract of the triterpenoids from seeds of the Neem tree (Azadirachta indica) can be fractionated rapidly and with economic use of solvents using the Biotage™ flash chromatography system. The fractions can be analysed by thin layer chromatography (TLC), or quantitatively, by supercritical fluid chromatography or high performance liquid chromatography (HPLC), for the determination of 11 tetranortriterpenoids, including the natural insecticide azadirachtin. After a second pass through the Biotage flash column, pure compounds, including azadirachtin and salannin can be obtained directly, although these compounds comprise less than 0.3% of the total mass of the seeds extracted and are present in a complex mixture of similar substances. Copyright © 1999 John Wiley & Sons, Ltd.
Source: Phytochemical Analysis
Institution: Keele University
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W2041538508