Work

Novel Design for Centrifugal Countercurrent Chromatography: Ii. Studies on Novel Geometries of Zigzag Toroidal Tubing

Year: 2010

Type: article

Abstract: The toroidal column using a zigzag pattern has been improved in both retention of the stationary phase and peak resolution. To further improve the retention of stationary phase and peak resolution, a series of novel geometric designs of tubing (plain, mid-clamping, flattened and flat-twisted tubing) was evaluated their performance in CCC. The results showed that the tubing which was flattened vertically against centrifugal force (vert-flattened tubing) produced the best peak resolution among them. Using vert-flattened tubing a series of experiments was performed to study the effects of column capacity and sample size. The results indicated that a 0.25 ml capacity column is ideal for analysis of small amount samples.

Source: Journal of Liquid Chromatography & Related Technologies

DOI: 10.1080/10826070903524100

Authors: Yi Yang, Haji Akber Aisa, Yoichiro Ito

Institutions: National Institutes of Health, Chinese Academy of Sciences, Xinjiang Technical Institute of Physics & Chemistry, National Heart, Lung, and Blood Institute, University of Chinese Academy of Sciences

Language: English

Topic: Chromatography in Natural Products

Subfield: Analytical Chemistry

Field: Chemistry

Domain: Physical Sciences

Open Access status: green

Funder: National Institutes of Health

Award: G4705346009