Work

Visible Fluorescence Detection of Type III Protein Secretion from Bacterial Pathogens

Year: 2010

Type: article

Abstract: Type III protein secretion is essential for many gram-negative bacterial infections of host cells and an attractive target for new antibacterial drugs. Here, we describe a bacterial protein effector-carboxypeptidase G2 (CPG2) reporter system for fluorescence and visible detection of type III protein secretion in Salmonella typhimurium. This system provides a general method for measuring protein expression and secretion as well as a high-throughput and quantitative assay for analyzing type III protein secretion inhibitors.

Source: Journal of the American Chemical Society

DOI: 10.1021/ja102257v

Authors: Jacob S. Yount, Lun K. Tsou, Paul D. Dossa, Amy L. Kullas, Adrianus W. M. van der Velden, Howard C. Hang

Institutions: Stony Brook University, Rockefeller University

Language: English

Topic: Escherichia coli research studies

Subfield: Endocrinology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funder: National Institute of Allergy and Infectious Diseases

Award: Northeast Biodefense Center