Work

162. Bioluminescence Imaging of a Tetanus Toxin C-Terminal Fusion Protein after Vector Delivery

Year: 2006

Type: article

Abstract: Firefly luciferase (FLuc) has routinely been used in vitro to quantitate gene expression by measuring the light emitted after theaddition of ATP and luciferin. It is now possible to replace luminometer-based analysis of luciferase activity with digital imaging techniques to measure luciferase activity noninvasively in cells and live animals over time. Previous studies have indicated that a single injection of an HSV amplicon vector (2 μl, 1 × 108 tu/ml) expressing Fluc cDNA under a viral promoter into the mouse cortex was sufficient to detect robust luciferase activity for at least one week.

Source: Molecular Therapy

DOI: 10.1016/j.ymthe.2006.08.185

Authors: Lisa Pike, Sam Wang

Institution: Massachusetts General Hospital

Language: English

Topic: bioluminescence and chemiluminescence research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $4,500