Non-invasive monitoring allograft rejection by simultaneous cellular and functional cardiac MRI
Year: 2009
Type: article
Abstract: An abdominal heterotopic working heart and lung transplantation model with DA to BN transplantation rat pairs was used. The transplanted hearts exhibit cardiac outputs and ventricular pressure similar to those of native hearts. In this model, mild (Grade 1A or B) rejection develops by post-operation day (POD) 2.5–3.5, Grade 2 rejection develops on POD 4.5–5.5, and moderate to the severe (Grade 3A) rejection develops after POD 6–7. ECG and respiration gated T 2 *-weighted cine imaging on Bruker AVANCE 4.7-T system was used for in-vivo imaging with an in-plane resolution of 156 μm. Tagged MRI is achieved with a modified DANTE sequence. Strains were analyzed by the HARP method with software obtained from Diagnosoft, Inc . Immune cells, mostly macrophages, are labeled in situ by direct intravenous injection of USPIO particles 1 day prior to MRI scans. Immune cells, mainly monocytes and macrophages, take-up circulating USPIO particles by endocytosis. Foci of labeled immune cells in the rejecting graft are observed by regions of hypointensity in T 2 *-weighted images (Fig. 1 ). The observed immune-cell infiltration is heterogeneous, thus it is not surprising to have incidences of false-negative results with biopsy. Monitoring immune-cell infiltration with MRI is not only non-invasive, but also provides whole-volume 3D perspective of rejection. T2*-weighted in vivo MRI for different allografts after USPIO administration . Areas with UPSIO-labeled macrophage infiltration show signal loss in both LV and RV. To discern if the heterogeneity of rejection is manifested in cardiac function, tagged MRI is used to monitor regional ventricular contractile function. Although global function may appear normal, the rejected allograft may show local hypokinesis (Fig. 2D ). Strain analysis by HARP was used to quantify the local contractile function. Regions with compromised strains (Fig. 2E & 2F ) largely correlate with the areas of higher macrophage infiltration. (A) T2*-weighted MRI for an allograft after USPIO administration . (B) Pseudo-coloring of different contrast-to-noise ratios (CNR) for easier visualization. (C, D) Tagged MRI of the same allograft heart at ED (C) and ES (D). The block arrows point to regions with compromised contractile functions. (E) Colored Ecc strain map of the allograft. (F) Ecc stran values of 48 probe-points throughout LV. For easier visualization of the regions with compromised strains, 48 probe-points were placed evenly throughout LV, starting at the anterior intercept of LV and RV (Fig. 2F & Fig. 3A ). The probe-point is considered "compromised" if the strain value of the particular location deviats from the mean isograft values by more than 1 standard deviation; otherwise the probe-point is scored as "normal" (Fig. 3B ). The number of compromised probe-points is well correlated with rejection grades (Fig. 3C ), which can potentially be useful clinical index for rejection. (A) Ecc strain values of 48 probe-points throughout LV for an isograft (triangle) and 2 allografts with Grade II (square) and Grade IV (circle) rejection . (B) Status of probe-points for 3 allografts with different rejection grades. Compromised probe-points are black, whereas the normal probe-points are left blank. (C) Degrees of compromised probe-points for different rejection grades. In our model, acute allograft rejection after heart transplantation is spatially heterogeneous, which is manifested in both immune-cell infiltration and ventricular function. Cardiac MRI is both non-invasive and provides 3D, whole-heart perspective of rejection status, which potentially allows more reliable detection of acute allograft rejection.
Authors: Yijen L Wu, Qing Ye, Kazuya Sato, Lesley M Foley, T Kevin Hitchens, Chien Ho
Institution: Carnegie Mellon University
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $3,000
OpenAlex ID: W2042365157