Work

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca 2+ Transients in Ciliated Tracheal Epithelial Cells

Year: 2003

Type: article

Abstract: To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca(2+) signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca(2+) transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca(2+) signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

Source: Infection and Immunity

DOI: 10.1128/iai.71.12.7208-7210.2003

Authors: Nathan A. Groathouse, Robert A. Heinzen, Scott Boitano

Institutions: University of Wyoming, Colorado State University, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, University of Arizona

Language: English

Topic: Bacterial Infections and Vaccines

Subfield: Microbiology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: National Institutes of Health, National Heart, Lung, and Blood Institute, National Center for Research Resources