Work

Inhibition by spermine of the induction of nitric oxide synthase in J774.2 macrophages: requirement of a serum factor

Year: 1994

Type: article

Abstract: Polyamines are endogenous regulators of various cell functions. Nitric oxide (NO) is a cytostatic and cytotoxic free radical which is produced by the inducible NO synthase (iNOS) in immuno-stimulated macrophages. We tested whether spermine modulates the induction of iNOS in J774.2 macrophages. Stimulation of macrophages by bacterial lipopolysaccharide (LPS) or gamma-interferon increased the accumulation of nitrite in the culture medium. Spermine (10(-6) - 10(-4) M) inhibited nitrite production without causing cytotoxicity. This inhibition of NO formation by spermine was significantly reduced when it was given 6 h after LPS. Spermine did not inhibit nitrite accumulation when foetal calf serum was omitted from the tissue culture medium. Thus, spermine is an inhibitor of the induction of iNOS, and its inhibitory activity requires the presence of a serum factor.

Source: British Journal of Pharmacology

DOI: 10.1111/j.1476-5381.1994.tb13078.x

Authors: Csaba Szabó, Garry J. Southan, Elizabeth Wood, Christoph Thiemermann, John R. Vane

Institutions: St Bartholomew's Hospital, Queen Mary University of London, William Harvey Research Institute

Language: English

Topic: Polyamine Metabolism and Applications

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2042834418

FWCI: 1.774

Cites: 8

Cited by: 75

Related to: 10