Work

The three yeast A kinases have specific signaling functions in pseudohyphal growth

Year: 1998

Type: article

Abstract: The three yeast A kinase catalytic subunit isoforms are redundant for viability. We demonstrate that they have dramatically different roles in pseudohyphal development: Tpk2 is essential, whereas Tpk3 inhibits. Tpk1 has no discernible effect. Two-hybrid analysis identified the transcription factor Sfl1 as a protein that interacts specifically with Tpk2, but not Tpk1 or Tpk3. Deletion of SFL1 enhances pseudohyphal and invasive growth. Flo11, a cell surface flocculin required for pseudohyphal development, is transcriptionally regulated by Tpk2 and Sfl1. Genetic evidence indicates that Tpk2 acts upstream of Sfl1 in the regulation of Flo11.

Source: Proceedings of the National Academy of Sciences

DOI: 10.1073/pnas.95.23.13783

Authors: Laura S. Robertson, Gerald R. Fink

Institutions: Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology

Language: English

Topic: Fungal and yeast genetics research

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: green

Funders: National Institutes of Health, National Human Genome Research Institute, National Institute of General Medical Sciences

Awards: MESSENGER RNA STRUCTURE IN TRANSLATIONAL CONTROL, The Role of Genetic Variation in the Fungal/Host Interaction, TRAINING GRANT IN GENOMIC SCIENCES, G6320805194