Work

Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes.

Year: 1993

Type: article

Abstract: We have recently shown that two proteins, proliferating cell nuclear antigen (PCNA) and p21, are associated with cyclin D. Here we show that PCNA and p21 are common components of a wide variety of cyclin/cyclin-dependent kinase complexes in nontransformed cells. These include kinase complexes containing cyclin A, cyclin B, and cyclin D, associated either with CDC2, CDK2, CDK4, or CDK5. We show that PCNA and p21 form separate quaternary complex with each cyclin/CDK and that these quaternary complexes contain a substantial, if not major, fraction of the cell cycle kinases in asynchronously growing cells. These results suggest that PCNA and p21 may perform a common function for all these kinases.

Source: Molecular Biology of the Cell

DOI: 10.1091/mbc.4.9.897

Authors: H Zhang, Y Xiong, D Beach

Institutions: Howard Hughes Medical Institute, Cold Spring Harbor Laboratory

Language: English

Topic: Cancer-related Molecular Pathways

Subfield: Oncology

Field: Medicine

Domain: Health Sciences

Open Access status: green

Funders: Howard Hughes Medical Institute, National Institutes of Health, National Institute of General Medical Sciences

Awards: MAMMALIAN CELL MITOTIC CHECKPOINT CONTROLS, G895310647