miR-34c attenuates epithelial-mesenchymal transition and kidney fibrosis with ureteral obstruction
Year: 2014
Type: article
Abstract: micro RNAs (miRNAs) are small non-coding RNAs that act as posttranscriptional repressors by binding to the 3'-UTR of target mRNAs. On the other hand, mesenchymal-epithelial transition (EMT) and kidney fibrosis is a pathological process of chronic kidney disease (CKD), and its relationship to miRNAs is becoming recognized as a potential target for CKD therapies. To find new miRNAs involved in EMT, we examined miRNA expression in experimental models of EMT and renal epithelialization using microarray, and found that miR-34c attenuates EMT induced by TGF-β in a mouse tubular cell line. To confirm the effects of miR-34c in vivo, we administered the precursor of miR-34c to mice with unilateral ureteral obstruction, and miR-34c decreased kidney fibrosis area and the expression of connective tissue growth factor, α-SMA, collagen type 1, collagen type 3 and fibronectin. In conclusion, our study showed miR-34c attenuates EMT and kidney fibrosis of mice with ureteral obstruction.
Source: Scientific Reports
DOI: 10.1038/srep04578
Institutions: Keio University, Keio University Hospital
Language: English
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $2,690
Awards: Mechanism of regeneration of renal tubular cells and differentiation from ES and iPS cells to renal tubular cells, The establishment of themethod to induce renal cellsfor renal disease specific iPS cell research, An investigation of mechanism of progression of kidney disease using renal tubular cells differentiated from ES/iPS cells
OpenAlex ID: W2043373020