Work

Canine enteric submucosal cultures: Transmitter release from neurotensin-immunoreactive neurons

Year: 1989

Type: article

Abstract: A culture system of dispersed submucosal neurons from canine ileum has been developed. The neuronal nature of over 80% of the cells in culture was confirmed by positive staining with a neurofilament antibody. In this culture system, neurotensin-immunoreactive neurons constituted greater than 50% of the total cell population. Neurotensin immunoreactivity in these cells was chromatographically characterized as a single molecular form coeluting with synthetic neurotensin (1-13). We have assessed the release of immunoreactive neurotensin by stimulatory and inhibitory transmitters, and by post-receptor activators of cell function. Forskolin (10 microM), the calcium ionophore A23187 (100 nM), and the active phorbol ester beta-12 myristrate 13-acetate (10 nM), each significantly increased neurotensin release compared with basal peptide secretion. The concomitant application of ionophore and phorbol ester resulted in a marked increase in neurotensin release and this stimulatory response was inhibited over 70% by somatostatin (100 nM). Substance P (0.1-100 nM) caused a dose-dependent increase in neurotensin release. Somatostatin (100 nM) reduced maximal stimulation with 100 nM substance P by 79%. Our results suggest that this submucosal culture system represents an entirely new model for characterizing transmitter release from enteric neurons.

Source: Neuroscience

DOI: 10.1016/0306-4522(89)90123-1

Authors: D.L. Barber, A.M.J. Buchan, S.E. Leeman, A.H. Soll

Institutions: Yale University, University of British Columbia, University of Massachusetts Chan Medical School, University of California, Los Angeles

Language: English

Topic: Neuropeptides and Animal Physiology

Subfield: Cellular and Molecular Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: hybrid

Estimated APC paid: $3,500