Work

Mitogenic Effect by Lipopolysaccharide and Pokeweed Lectin on Density-inhibited Chick Embryo Fibroblasts

Year: 1973

Type: article

Abstract: The B lymphocyte mitogens, bacterial lipopolysaccharide (LPS), pokeweed lectin, and tuberculin, induced proliferation in density-inhibited monolayer cultures of chick embryo fibroblasts. The stimulation was seen both as an early increase in sugar uptake and cell volume and later as an increase in thymidine incorporation and cell number. The concentration of LPS maximally stimulating fibroblasts was remarkably low, about 0.1-1 ng/ml. LPS preparation with very different sugar chains gave quite similar results indicating that the architecture of the hydrophilic carbohydrate part is not critical for the mitogenic effect.

Source: The Journal of Experimental Medicine

DOI: 10.1084/jem.138.6.1356

Authors: Antti Vaheri, Erkki Ruoslahti, Matti Sarvas, Marjatta Nurminen

Institution: University of Helsinki

Language: English

Topic: Immune Response and Inflammation

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: bronze

OpenAlex ID: W2044219108