Work

Selective Depletion of rRNA Enables Whole Transcriptome Profiling of Archival Fixed Tissue

Year: 2012

Type: article

Abstract: We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.

Source: PLoS ONE

DOI: 10.1371/journal.pone.0042882

Authors: John D. Morlan, Kunbin Qu, Dominick V. Sinicropi

Institution: Genomic Health (United States)

Language: English

Topic: Genomics and Phylogenetic Studies

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,382